To run a centrifuge correctly, you should:


Balance tubes by spacing them equally around the centrifuge and Always balance tubes with other tubes containing a same volume of liquid are right. 
If you don't space them out equally, you will have a lot of broken glass to clean up...trust me. The same thing can happen if you don't have equal amounts of liquid in each tube, but it doesn't have to be exactly the same in every one.

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